|Synonyms||FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1|
|Molecular Weight||Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.|
|AA Sequence||KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF|
|Purity||> 95 % by SDS-PAGE and HPLC analyses.|
|Biological Activity||Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.|
|Physical Appearance||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Formulation||Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1 % Tween 80.|
|Endotoxin||Less than 1 EU/μg of rHuIL-36α, 153a.a. as determined by LAL method.|
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|Usage||This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.|
|Reference||1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25.|
2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973.
3. Barksby HE, Lea SR, Preshaw PM, et al. 2007. Clin Exp Immunol. 149:217-25.
4. Smith DE, Renshaw BR, Ketchem RR, et al. 2000. J Biol Chem. 275:1169-75.
5. Dunn E, Sims JE, Nicklin MJ, et al. 2001. Trends Immunol. 22:533-6.
|Background||Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha (153 a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57 – 68 % a.a. sequence identity with mouse, rabbit, equine and bovine IL-36α and 27 – 57 % a.a. sequence identity with other novel IL-1 family members.|