Protein A Affinity Resin

PrimeGene® now offers Protein A resin---Protein A Sepharose CL-4B! It is designed to significantly reduce the cost of the Protein A capture step, offer a standardized platform for bioprocessing applications and meet the industry's demands for quality and supply expectations.

Reliable Performance:

  • High dynamic binding capacity, and low protein A leaching
  • Reduction in protein A resin cost
  • An animal free fermentation process
  • Manufacturing process that meets ISO9001:2008 quality system standards
  • Security supply chain

Performance Specifications:

  • ◆ Ligand:Recombinant Staphylococcus Protein A (rSPA) expressed in E.coli, which contains all five immunoglobin binding domains (E,D,A,B,C).
  • ◆ Bead structure: 4% cross-linked agarose
  • ◆ Particle size: 45–165 μm
  • ◆ Mean bead size: 90 μm
  • ◆ Ligand density: ~5 mg protein A/ml medium
  • ◆ Available capacity: >30 mg human IgG/ml medium
  • ◆ Protein A leaching: <10 ng/mg IgG
  • ◆ Caustic Stability: 100 cycles with 0.1M NaOH
  • ◆ Recommended FlowVelocity: 20-150 cm/h
  • ◆ Recommended pH: Long term: 3–9, Short term: 2–10
Protein A Affinity Bottled Resin
Cat. # Product Name Size A Size B Size C Size D
110107A
Protein A ResinCL-4B
5ml 25mg 200ml 1000ml
Pre-Packed Protein A Columns
Cat. # Product Name Size 
120107A1B Protein A Separose CL-4B Column, 1ml, 2 columns
2x1ml
120107A1E
Protein A Separose CL-4B Column, 1ml, 5 columns
5x1ml

120107A5A
Protein A Separose CL-4B Column, 5ml, 1 column
1x5ml

120107A5E
Protein A Separose CL-4B Column, 5ml, 5 columns
5x5ml

Purification Ligands for Bioprocessing
---Protein A, Protein G, Protein L, Protein A/G, Protein A/G/L

Key Advantages
  • Broad Product Series
  • High purity: >97%
  • High Lot to Lot consistency and low endotoxin (<0.1 EU/ug )
  • An animal free fermentation process
  • Manufacturing process that meets ISO9001:2008
  • Security supply chain
Protein A

Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series. It binds the heavy chain within the Fc region of most immunoglobulins. It is ideal for purification of polyclonal or monoclonal IgG antibodies.

Cat. # Product Name Description Size A Size B Size C Special Order
1001-01 Protein A,His Protein A with His-tag at N-terminus 10mg 100mg 1g Inquiry
1001-02 Protein A-Cys, His Protein A with His-tag at N-terminus, Cys residue at C-terminus 10mg 100mg 1g Inquiry
1001-04 Protein A-Cys Protein A with Cys residue at C-terminus 10mg 100mg 1g Inquiry
1001-05 Protein A 5 IgG-binding domains 10mg 100mg 1g Inquiry
1001-07 Protein A,Long Form 5 IgG-binding domains and cell membrane binding region,similar to native Protein A 10mg 100mg 1g Inquiry
1001-09 Protein A-Cys,Long Form 5 IgG-binding domains and cell membrane binding region,similar to native Protein A,Cys residue at C-terminus 10mg 100mg 1g Inquiry
Protein G

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. The recombinant Protein G is a genetically engineered protein containing 3 immunoglobulin-binding regions of protein G. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG.

Cat. # Product Name Description Size A Size B Size C Special Order
1002-01 Protein G-His Contains 3 immunoglobulin -binding regions with His-tag at C-terminus 1mg 10mg 1g Inquiry
1002-03 Protein G Contains 3 immunoglobulin -binding regions 1mg 10mg 1g Inquiry
1002-04 Cys-Protein G Contains 3 immunoglobulin -binding regions with Cys residus at N-terminus 1mg 10mg 1g inquiry
Protein A/G

The recombinant Protein A/G is a genetically engineered protein containing 5 immunoglobulin -binding regions of protein A and 2 of protein G. The binding capacity of Protein A/G is broader than either Protein A or Protein G alone. The extended Fc-binding properties of Protein A/G make it popular tool in the purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD.

Cat. # Product Name Description Size A Size B Size C Special Order
1003-01 Protein A/G-His Contains 7 immunoglobulin -binding regions with His-tag at C-terminus 1mg 5mg 1g Inquiry
1003-02 Protein A/G Contains 7 immunoglobulin -binding regions 1mg 5mg 1g Inquiry
1003-03 Cys-Protein A/G Contains 7 immunoglobulin -binding regions with Cys residus at N-terminus 1mg 10mg 1g Inquiry
1003-04 Protein A/G-Cys Contains 7 immunoglobulin -binding regions with Cys residus at C-terminus 1mg 5mg 1g Inquiry
Protein L

Recombinant Protein L contains 5 kappa-binding domain. Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins.

Cat. # Product Name Description Size A Size B Size C Special Order
1004-01 Protein L-His Contains 5 immunoglobulin -binding regions with His-tag at N-terminus 1mg 10mg 1g Inquiry
1004-02 Protein L-Cys,His Contains 5 immunoglobulin -binding regions with His-tag at N-terminus and Cys residus at C-terminus 1mg 10mg 1g inquiry
1004-03 Protein L-Cys Contains 5 immunoglobulin -binding regions with Cys residus at C-terminus 1mg 10mg 1g inquiry
1004-04 Protein L Contains 5 immunoglobulin -binding regions 1mg 10mg 1g inquiry
1004-05 Cys-Protein L Contains 5 immunoglobulin -binding regions with Cys residus at N-terminus 1mg 10mg 1g inquiry
Protein A/G/L

Recombinant fusion protein A/G/L contains five Ig-binding regions of protein L, five IgG binding domains from Protein A, and two Ig-binding region of protein G. Protein A/G/L binds to all human IgG subclasses, IgA, IgE, IgM and IgD;

Cat. # Product Name Description Size A Size B Size C Special Order
1006-01 Protein A/G/L Contains 12 immunoglobulin -binding regions 1mg 5mg 1g inquiry
1006-02 Protein A/G/L-Cys Contains 12 immunoglobulin -binding regions with Cys residus at C-terminus 1mg 5mg 1g inquiry
1006-03 Cys-Protein A/G/L Contains 12 immunoglobulin -binding regions with Cys residus at N-terminus 1mg 10mg 1g inquiry

Table of Binding Capability



Protein A Protein G Protein L Protein A/G Protein A/G/L
Human IgG
+++ +++ +++ +++ +++

IgG1
++++ ++++ ++++ ++++ ++++

IgG2
++++ ++++ ++++ ++++ ++++

Ig3

+++ +++ +++ +++

IgG4
++++ ++++ ++++ ++++ ++++

IgA
+
+++ + ++++

IgA1
+
+++ + ++++

IgA2
+
+++ + ++++

IgD


+++
++++

IgE
++
+++ ++ ++++

IgM
+
+++ + ++++

Fab
+ + +++ + ++++

ScFv
+
+++ + ++++
Mouse IgG
++ ++ +++ +++ ++++

IgG1
+ ++++ +++ +++ ++++

IgG2a
++++ ++++ +++ ++++ ++++

IgG2b
+++ +++ +++ +++ +++

IgG3
++ +++ +++ +++ +++

IgM


+++
+++
Rat IgG
+ ++ +++ ++ +++

IgG1

+ +++ ++ +++

IgG2a

++++ +++ +++ ++++

IgG2b

++ +++ + +++

IgG2c
++ ++ +++ +++ +++

IgG3
+ ++ ? ++ ++
Horse IgG
++ ++++ ? ++++ ++++

IgG(ab)
+
? + +

IgG(c)
+
? + +

IgG(T)

+++ ? +++ +++
Goat IgG
++ +++
++++ ++++

IgG1
+ +++
+++ +++

IgG2
+++ +++
+++ +++
Guinea-pig IgG
+++ + ? +++ +++

IgG1
++ + ? ++ ++

IgG2
++ + ? ++ ++
Sheep IgG
+ ++
+++ +++

IgG1
+ ++
+++ +++

IgG2
+++ +++
+++ +++
Cow IgG
+ +++
+++ +++

IgG1
+ +++
+++ +++

IgG1
++ +++
+++ +++
Rabbit IgG
+++ +++ + +++ +++
Cat IgG
+++ + ? + +++
Dog IgG
++ + ? +++ +++
Pig IgG
+++ ++ +++ +++ +++
Chicken IgY





Monkey IgG
++++ ++++ ? ++++ ++++
Hamster IgG
+ ++ +++ ++ ++
Koala IgG

+ ? + +
Llama IgG

+ ? + +
Donkey IgG
++ +++ ? +++ +++

+: binding strength, ?: not tested

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