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Recombinant Human Interleukin-10 GMP

rHuIL-10 GMP

Item No:GMP-101-10
Price:
$ 119
Size:
Amount:
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Technical Data
    Source
    Escherichia coli
    Molecular Weight
    Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
    Sequence
    SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
    Purity
    > 95% by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 U/mg.
    Physical Appearance
    Sterile filtered white lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH 6.0.
    Endotoxin
    Less than 0.01 EU/µg of rHuIL-10 GMP as determined by LAL method.
    Host Cell Protein
    Less than 0.10% when tested by ELISA.
    Reconstitution
    Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    - Refer to lot-specific CoA for the Expiry Date.
    Usage
    This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory, or further evaluation purposes. NOT FOR HUMAN USE.
    Quality Statement
    The manufacturing and testing of these products comply with ICH Q7 guidelines.
    Background
    Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.
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