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Recombinant Human Insulin-like Growth Factor-Binding Protein 5

rHuIGF-BP5

Item No:105-01B5
Technical Data
    Accession
    P24593
    GeneID
    3488
    Source
    Escherichia coli.
    Molecular Weight
    Approximately 28.6 kDa, a single non-glycosylated polypeptide chain containing 252 amino acids.
    AA Sequence
    LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE
    Purity
    > 96 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0.
    Endotoxin
    Less than 0.1 EU/μg of rHuIGF-BP5 as determined by LAL method.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - 12 months from date of receipt, -20 to -70 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Usage
    This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
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