Other specifications inquiry
Item No.
Product Name
Size
Amount
End User or Agent
Unit Name
Company Website
Contacts
Mobile
Email
Country/Region

Recombinant Human Basic Fibroblast Growth Factor

rHubFGF

Item No:104-02
Technical Data
    Synonyms
    FGF-2, HBGF-2
    Accession
    P09038
    GeneID
    2247
    Source
    Escherichia coli
    Molecular Weight
    Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
    Size
    10 µg/100 µg/500 µg/1 mg
    Sequence
    MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
    Purity
    > 96% by SDS-PAGE analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 U/mg.
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.6.
    Endotoxin
    Less than 1 EU/µg of rHubFGF as determined by LAL method.
    Reconstitution
    Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/ml. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Usage
    This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
    SDS-PAGE
    Reference
    1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.
    2. Gospodarowicz D. 1974. Nature. 249:123-7.
    3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.
    4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.
    5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.
    6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.
    7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.
    Background
    Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human bFGF shares 54% amino acid sequence identity with aFGF. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.
Do you want to learn more about our product?
Try our product selector
Screening Products
Similar Product Recommendations
Contact Us
Service Hotline:400-821-3475
Address: Building 16, No. 3879 Dongchuan Road, Minhang District, Shanghai, 200245, P.R. China
Zip Code:200245
Phone:021-61072066
Order E-mail:order.PG@bio-techne.com
Inquiry E-mail:info.PG@bio-techne.com
COA Application
Thank you for your support and trust in PrimeGene, please enter the Lot No. and Catalog No. for inquiry.
Size:
Contact Us

Service Hotline:400-821-3475

Inquiry E-mail:order.PG@bio-techne.com

Inquiry E-mail:info.PG@bio-techne.com

Support E-mail:techsupport.PG@bio-techne.com

Dealer inquiry
BeiJing
ShangHai
ChongQing
JiangSu
ZheJiang
GuangDong
AnHui
TianJin
HeBei
ShanXi
NeiMengGu
LiaoNing
JiLin
HeiLongJiang
FuJian
JiangXi
ShanDong
HeNan
HuBei
HuNan
GuangXi
HaiNan
SiChuan
GuiZhou
YunNan
XiZang
ShanXi
GanSu
QingHai
NingXia
XinJiang
XiangGang
AoMen
TaiWan
Search Results

Delivery

Price Inquiry

Technical Consulting